Abstract
Agar is a commercially important biopolymer by marine algae and used up in various industries like microbiological, food, medicine, cosmetics. Acid based pretreatment is explored in this study for open vessel and steam boiler methods in terms of agar production at wet conditions. For the selection of acids the very strong (H2SO4), strong (HCl) and weak acid (CH3COOH) were used and CH3COOH was selected. Furthermore, the strength of CH3COOH was optimized at 1%. The steam boiler treatment with 1h soaking in 1% CH3COOH produced 10g agar which was better than open vessel treatment.
References
Bixler, H.J. and H. Porse. 2011, A decade of change in the seaweed hydrocolloids industry. J. Appl. Chem. 23: 321-335.
Fidelis, G.P., R.B.G. Camara, M.S.S.P. Queiroz, P.C. Santos and H.A.O. Rocha. 2014. Proteolysis, and ultrasound enhanced extraction of anticoagulant and antioxidant sulfated polysaccharides from the edible seaweed, Gracilaria birdiae. Molecules. 19(11): 18511-18526.
Phan, T.D., Debeaufort, F., Voilley,A.,& Luu,D.(2009). Biopolymer interactions affect the functional properties of edible films based on agar, cassava and arabinoxylan blends. Journal of food Engineering, 90, 548-558.
Praiboon, J., A. Chirapart, Y. Akakabe, O. Bhumibhamon and T. Kajiwara. 2006. Physical and chemical characterization of agar polysaccharide extracted from the Thai and Japanese species of Gracilaria. Science Asia. 32(suppl. 1): 11-17.
Rochas, C. and M. Lahaye. 1989. Average molecular weight and molecular weight distribution of agarose and agarose–type polysaccharides. Carbohydrate polymers. 10: 289-298.
Sousa, A. and M.P. Goncalves. 2015. The influence of locust bean gum on native and alkali- modified agar gels. Food Hydrocolloids. 44: 461-470.
Villanueva, R., A. Sousa, M.P. Goncalves and L. Hilliou. 2010. Production and properties of agar from the invasive marine alga, Gracilaria vermiculophylla (Gracilariales, Rhodophyta). J. Appl. Phycol. 22(2): 211-220.
Yang, M.Y., J.D. Dong and M.S. Kim. 2012. Taxonomic notes on five species of Gracilariaceae from Hainan, China. Algae. 27(3): 175-187.
Yousefi, M.K., H.R. Islami and Y. Filizadeh. 2013. Effect of extraction process on agar properties of Gracilaria corticata (rhodophytra) collected from the Persian Gulf. Phycologia. 52(6): 481-487.

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Copyright (c) 2017 Fareeha Pervaiz, Asma Tabassum, Sofia Qaisar, Sidra Nadeem, Aijaz Panhwar, Aliya Rehman