Pakistan Journal of Marine Sciences <p><strong>Aim and Scope</strong></p> <p>The Pakistan Journal of Marine Sciences (Print ISSN 1019-8415, Online ISSN 2710-4583, CODEN PJMSE8) a bi-annual journal is an official publication of The Marine Reference Collection and Resource Centre, University of Karachi.</p> <p>PJMS is dedicated to publish original research papers in the broad field of Ocean Sciences. All the major aspects of marine science are covered in PJMS including zoology, botany, geology, chemistry, physiology, systematic, ecology, physical oceanography and fisheries. The journal is abstracted and indexed by ASFA, BIOSIS, PASTIC, Chemical Abstracts, Current Contents, Oceanic Abstracts and Zoological Records.</p> Marine Reference Collection and Resource Centre, University of Karachi en-US Pakistan Journal of Marine Sciences 1019-8415 AUTHENTICATION AND TRACEABILITY OF PUFFERFISH ‎SPECIES USING DNA SEQUENCING <p>Pufferfish is known a powerful toxin in its skin and organs called Tetrodotoxin. Validation of food authenticity is usually based on the analysis of proteins or DNA sequences. DNA based methods are more effective and can also be applied to different food matrices. DNA barcoding is a rapidly rising global initiative in which species are characterized using mtDNA sequence. This is based on the premise that species are generally well delineated by a species-specific sequence. In this study, we revealed DNA barcoding of three pufferfish species <em>Lagocephalus sceleratus</em>, <em>Sphoeroides pachygaster </em>and <em>Torquigener flavimaculosus</em> using the mtDNA cytochrome b gene region. There were 122 variable and 266 conservative nucleotides of which 110 were parsimony informative over 388 bp sequences. The mean nucleotides composition was found as %28.0 thymine (T), %30.7 cytosine (C), %24.8 adenine (A) and %16.6 guanine (G). Twelve haplotypes were found out of 18 sequences and mean haplotype diversity was observed at 0.9346. This study has strongly authenticated and traceability the efficacy of Cty b in identifying <em>L. sceleratus, S. pachygaster </em>and<em> T. flavimaculosus</em> species with designated barcodes. This is the recorded of <em>L. sceleratus, S. pachygaster </em>and<em> T. flavimaculosus</em> cytochrome b gene sequence from the eastern Mediterranean coast of Turkey.</p> Servet Ahmet Doğdu Cemal Turan Copyright (c) 2021 Servet Ahmet Doğdu, Cemal Turan 2021-06-30 2021-06-30 30 1 1 11 FORM FACTOR OF JAPANESE THREADFIN BREAM, ‎NEMIPTERUS JAPONICUS (BLOCH, 1791)‎ <p>Japanese threadfin bream, <em>Nemipterus japonicus</em> is a large-scale commercial fish found in the Pacific and Indian Ocean. Form factor (<em>a<sub>3.0</sub></em>) is used widely for comparison of the fish body shape between geographic locations. The present study was aimed to estimate the form factor of <em>N. Japonicus </em>from available literature based on empirical model. Assessed <em>a</em><em><sub>3.0</sub></em> ranged from 0.0035 to 0.0270. The median (0.0143) and 95% confidence limit (0.0130-0.0168) indicated that <em>N. Japonicas </em>poses the typical torpedo body shape ‘fusiform’. Findings on the form factor of <em>N. japonicus</em> will be effective for perceiving further body shape alteration between geographic distributions.</p> Md. Ashekur Rahman‎ Jun Ohtomi Copyright (c) 2021 Md. Ashekur Rahman, Jun Ohtomi 2021-06-30 2021-06-30 30 1 13 19